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- Negative, normal flora present
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- Female: Sterile swab or wire loop is inserted into the cervical canal to obtain secretions or endocervical cell scrapings
- Male: A sterile cotton swab is used to collect secretions from the penile discharge
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- To identify the pathogenic organism that causes abnormal discharge and inflammation of the vagina or male urethra
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- Recent urination
- Recent douching
- Improper collection technique
- Contamination of the specimen
- Antibiotic administration
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